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[1]王玉巧,赵一玫,王凯*.复方止咳颗粒中盐酸麻黄碱含量的测定[J].武汉工程大学学报,2011,(01):19-21.[doi:10.3969/j.issn.16742869.2011.01.005]
　WANG Yu qiao,ZHAO Yi mei,WANG Kai.Determination of berberine hydrochloride in Zhike complex granules by RPHPLC[J].Journal of Wuhan Institute of Technology,2011,(01):19-21.[doi:10.3969/j.issn.16742869.2011.01.005]
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《武汉工程大学学报》[ISSN:1674-2869/CN:42-1779/TQ]

卷:
期数:
2011年01期
页码:
19-21
栏目:
化学与化学工程
出版日期:
2011-01-30

文章信息/Info

Title:
Determination of berberine hydrochloride in Zhike complex
granules by RPHPLC
文章编号:
16742869(2011)01001903
作者:
王玉巧1; 2; 赵一玫2; 王凯1*
1.武汉工程大学绿色化工过程教育部重点实验室,湖北 武汉 430074;
2.武汉红桃开国家企业技术中心,湖北 武汉 430074
Author(s):
WANG Yuqiao;  ZHAO Yimei;  WANG Kai
1.Key Laboratory for Green Chemical Process of Ministry of Education, Wuhan Institute of Technology,
Wuhan 430074, China;2.Statelevel Enterprise Technology Center of Wuhan RHK,Wuhan 430074, China
关键词:
复方止咳颗粒; 盐酸麻黄碱; RPHPLC; 色谱
Keywords:
zhike complex granules;  berberine hydrochloride;  RPHPLC;  chromatograph
分类号:
TQ460.7
DOI:
10.3969/j.issn.16742869.2011.01.005
文献标志码:
A
摘要:
建立RP-HPLC法测定复方止咳颗粒中盐酸麻黄碱的含量方法.采用Kromasil C18柱,流动相：乙腈-0.02 mol·L-1磷酸二氢钾溶液(含0.4%三乙胺,磷酸调pH至2.7）,流速：1.0 mL·min-1,检测波长210 nm.盐酸麻黄碱在0.1～2.0 μg范围内有良好的线性关系,r=0.999 8,平均回收率为100.3%,RSD为0.79%.结果表明:本方法简便快捷,结果准确,稳定性好,重现性好;同时,采用该方法对于自制的10个批号的复方止咳颗粒中盐酸麻黄碱的标示含量进行测定,结果为86.3%～96.0%.
Abstract:
A HPLC method was established for the determination of berberine hydrochloride in Zhike complex granules by RPHPLC. A Kromasil C18 column was used with acetonitrile and 0.02 mol·L-1 KH2PO4(595)（Including 0.4% triethylamine, adjust pH to 2.7 with phosphoric acid）as the mobile phase at the flowing rate of 1.0 mL·min-1.The detection wavelength was set at 210 nm. The calibration range of berberine hydrochloride was 0.1-2.0 μg(r=0.999 9). The mean recovery was 100.3% and RSD was 0.79%. The method is simple, rapid, accurate, reliable and reproducible. At the same time, using this method for the 10 batches of ephedrine hydrochloride of Zhike complex granules, the determined content is 86.3%-96.0%.

参考文献/References:

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［7］孙雪妹,杨明华,戴影秋.良园枇杷叶膏中盐酸麻黄碱的HPLC测定［J］.中国医药工业杂志,2006,37(11):768769.
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备注/Memo

备注/Memo:
收稿日期：20100709作者简介：王玉巧（1973）,女,湖北武汉人，硕士研究生.研究方向:中药新药质量标准研究制定.指导老师：王凯，副教授，博士.研究方向：新药及其中间的研究与开发、工艺革新.
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